Journal
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Volume 13, Issue 8, Pages 7882-7893Publisher
ESG
DOI: 10.20964/2018.08.57
Keywords
Cyclic voltammetry techniques; spectrophotometric methods; polyphenols; Thymus vulgaris; antioxidant capacity
Categories
Funding
- Algerian Ministry of Higher Education and Scientific Research (MESRS, DGRSDT)
- Algerian Thematic Agency for Scientific Research in Health (ATRSS)
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The present study aims to investigate the antioxidant activity of Thymus vulgaris extracts evaluated by DPPH, ABTS, chelating activity on ferrous ions tests, and evaluation of antioxidant capacity by electrochemical techniques (Cyclic Voltammetry Techniques CVT). Total polyphenols and flavonoids contents were quantified by spectrophotometric using Folin-Ciocalteu and FeCl3 reagents, respectively. The results showed that the total polyphenols contents and flavonoids contents were in the order MetE > EAE > ChE > BolE > AqE > PEE. The DPPH activity showed that IC50 values were ordered as follows: MetE (8.49 +/- 0.02 mu g/ml), BolE (16.98 +/- 0.05 mu g/ml), EAE (21.61 +/- 0.22 mu g/ml), ChE (26.01 +/- 0.08 mu g/ml), AqE (42,21 +/- 0.68 mu g/ml and PEE (149.1 +/- 0.52 mu g/ml). The ABTS+ assay revealed an important antioxidant activity of the extracts. The capacity of the metal chelating of MetE = 17 +/- 2 mu g/ml, EAE = 124 +/- 1 mu g/ml, ChE = 142 +/- 1 mu g/ml, BolE = 185 +/- 1 mu g/ml, AqE = 250 +/- 4 mu g/ml and PEE = 527 +/- 1 mu g/ml. Antioxidant activity measured by cyclic voltammetry method present important activity were in the order MetE > EAE > ChE > BolE > AqE > PEE. All methods showed a correlation between antioxidant activity and phenolics and/or flavonoids content. Finally these results clearly show that Thymus vulgaris possesses important antioxidant activity and can serve as potential source of bioactive healthy compounds that consumption could be useful in the prevention and/or treatment of diseases where reactive oxygen species and free radicals are involved.
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