Journal
GENE
Volume 555, Issue 2, Pages 178-185Publisher
ELSEVIER
DOI: 10.1016/j.gene.2014.11.001
Keywords
Zma-miR169; ZmNF-YA; Transient expression; Expression pattern; Abiotic stress; Maize leaves
Categories
Funding
- National Key Basic Research Program [2014CB138200]
- National High Technology Research and Development Program of China [2012AA10A306]
- National Natural Science Foundation of China [31271801]
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The miR169 miRNA family is highly conserved in plants. Its members regulate the expression of genes encoding the universal transcription factor subunit NUCLEAR FACTOR-Y subunit A (NF-YA) via transcript cleavage. NF-YA regulates gene expression by binding the CCAAT box sequence in target promoters. The miR169/NF-YA module plays a critical role during plant development and in plant responses to abiotic stress. We characterized the secondary structures of maize pre-miR169 miRNAs and predicted their potential gene targets. Coexpression of zma-miR169 and ZmNF-YA in Nicotiana benthamiana demonstrated that mutations in or deletion of target sites abolished regulation by zma-miR169. Maize seedlings were subjected to short-term (0-48 h) and long-term (15 days) drought, abscisic acid (ABA), or salt stress. Long-term exposure to PEG (drought stress) or NaCl (salt stress) repressed seedling growth. We investigated the expression patterns of zma-miR169s and their target ZmNF-YA genes in maize leaves and found diverse changes in expression in response to the three stress treatments. The expression of most zma-miR169 genes was downregulated by PEG and upregulated by ABA. In response to salt stress, zma-miR169 genes were upregulated initially and subsequently downregulated. Most ZmNF-YA genes were upregulated during the short term and downregulated by 15 days in response to the three stress treatments. (C) 2014 Elsevier B.V. All rights reserved.
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