Journal
GENE
Volume 568, Issue 1, Pages 40-49Publisher
ELSEVIER
DOI: 10.1016/j.gene.2015.05.004
Keywords
Peanut; Annexin genes; Cloning; Expression analysis
Categories
Funding
- National Natural Science Foundation of China [31471523]
- 948 Project [2013-Z65]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [2012302110002]
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Annexin, Ca2+ or phospholipid binding proteins, with many family members are distributed throughout all tissues during plant growth and development Annexins participate in a number of physiological processes, such as exocytosis, cell elongation, nodule formation in legumes, maturation and stress response. Six different full-length cDNAs and two partial-length cDNAs of peanut (AnnAh1, AnnAh2, AnnAh3, AnnAh5, AnnAh6, AnnAh7, AnnAh4 and AnnAh8) encoding annexin proteins, were isolated and characterized using a RT-PCR/RACE-PCR based strategy. The predicted molecular masses of these annexins were 36.0 kDa with acidic pis of 5.97-8.81. ANNAh1, ANNAh2, ANNAh3, ANNAh5, ANNAh6 and ANNAh7 shared sequence similarity from 35.76 to 66.35% at amino acid level. Phylogenetic analysis revealed their evolutionary relationships with corresponding orthologous sequences in soybean and deduced proteins in various plant species. Real-time quantitative assays indicated that these genes were differentially expressed in various organs. Transcript level analysis for six annexin genes under stress conditions showed that these genes were regulated by drought, salinity, heavy metal stress, low temperature and hormone. Additionally, the prediction of cis-regulatory element suggested that different cis-responsive elements including stress- and hormone-responsive-related elements could respond to various stress conditions. These results indicated that members of AnnAhs family may play important roles in the adaptation of peanut to various environmental stresses. (C) 2015 Elsevier B.V. All rights reserved.
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