Journal
GENE
Volume 563, Issue 1, Pages 17-23Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2015.02.076
Keywords
VE-cadherin; MRTF-A; p300; Transcription regulation
Categories
Funding
- National Natural Science Foundation of China [31171303, 31171297, 31270837]
- Program for Changjiang Scholars and Innovative Research Team in University of the Ministry of Education of China [IRT1166]
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Vascular endothelial cadherin (VE-cadherin) is the major determinant of endothelial cell contact integrity and is required in vascular development and angiogenesis. Serum response factor (SRF) plays essential roles in postnatal retinal angiogenesis and adult neovascularization. It is unclear whether transcription of VE-cadherin is mediated by a SRF co-activator, myocardin-related transcription factor-A (MRTF-A): Here we have demonstrated that MRTF-A is a key regulatory factor to activate the transcription of VE-cadherin in human umbilical vein endothelial cells (HUVECs). siRNA-mediated knockdown of MRTF-A decreased the level of VE-cadherin in HUVECs. Vascular endothelial growth factor (VEGF) induced MRTF-A binding to the SRF-binding site (CArG box) within VE-cadherin promoter. Histone acetyltransferase p300 and MRTF-A could synergistically augment the expression of VE-cadherin by enhancing acetylation of histone3K9 (H3K9Ac), histone3K14 (H3K14Ac) and histone4 at the SRF-binding site within VE-cadherin promoter. Taken together, these data identified a detailed regulatory mechanism of VE-cadherin gene expression. (C) 2015 Elsevier B.V. All rights reserved.
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