Potential application of developed methanogenic microbial consortia for coal biogasification

Microbially enhanced coalbed methane has become an important research topic in the later years. The biological conversion of coal to methane can be conceived as a feasible and environmental friendly approach for improving coalbed methane production. Within the strategies for stimulation of gas production, the addition of a microbial consortium or bioaugmentation can be seen as a promising alternative. However, relatively few studies have been conducted on the strategies for enriching microbial population ex-situ under initial atmospheric exposure for subsequent injection into coal seams to stimulate biodegradation and methanogenesis. The development of methanogenic microbial consortia, especially those that can tolerate moderate and low oxygen concentrations and still retain anaerobic functionality, can be considered as an attractive biological complement for coal biogasification. The performance of promising microbial consortia was evaluated at low concentrations of nutrient amendments (e.g., 22.4% v/v, 3.36 mg/cm(3) TSB) and [NaCl] 6.6 mg/cm(3) as a possible scenario and to foresee the elevated costs of nutrient utilization at large-scale operations (i.e., in-situ and/or ex-situ applications). Incubation periods of up to four months were tested at 23 degrees C. Headspace concentrations of CH4 and CO2 were analyzed by gas chromatography. After 61 days of incubation for the most promising microbial samples, generated headspace gas concentrations reached 95,700 ppm (14 sft(3)/ton) for methane and 37,560 ppm (5.5 sft(3)/ton) for carbon dioxide. Microbial diversity in promising consortia was investigated. Both bacteria and archaea were identified.