Journal
GENE
Volume 561, Issue 1, Pages 115-123Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2015.02.013
Keywords
Bat; RIG-I; STAT-1; IFN-beta; VSV
Categories
Funding
- National Basic Research Program of China [2012CB910400]
- National Natural Science Foundation of China [81172816, 81272369]
- Science and Technology Commission of Shanghai Municipality [11DZ2260300]
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Wild Chinese horseshoe bats have been proven to be natural reservoirs of SARS-like coronaviruses. However, the molecular characterization of key proteins in bats still needs to be explored further. In this study, we used cloning and bioinformatics to analyze the sequence of RIG-I, STAT-1 and IFN-beta in the immortalized cell lines from Rhinolophus affinis and Rhinolophus sinicus. Then, we treated different bat cells, mouse embryonic fibroblasts (MEF) and splenocytes with polyinosinic-polycytidylic acid (polyI:C) and vesicular stomatitis virus (VSV) to assess and compare antiviral immune responses between bats and mice. Our results demonstrated that bat RIG-I, STAT-1 and IFN-beta showed close homology with human, mouse, pig and rhesus monkey. RIG-I and STAT-1 were both highly expressed in bat spleen. Furthermore, IFN-beta was induced by polyI:C and VSV in both bat and mouse cells. These findings have provided new insight into the potential characteristics of the bat innate immune system against viral infection. (C) 2015 Elsevier B.V. All rights reserved.
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