Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 143, Issue 1, Pages 160-166Publisher
WILEY
DOI: 10.1002/ijc.31383
Keywords
lung cancer; cell culture; epithelial cells; basal cells; stem/progenitor cells
Categories
Funding
- Cancer Research UK [C11496/A17786]
- CRUK Lung Cancer Centre of Excellence
- Roy Castle Lung Cancer Foundation
- Wellcome Trust [WT209199/Z/17, FC001169, FC001202]
- Stand Up To Cancer (SU2C)
- Rosetrees Trust
- NovoNordisk Foundation [16584]
- Prostate Cancer Foundation
- Breast Cancer Research Foundation (BCRF)
- European Research Council (THESEUS)
- Marie Curie Network PloidyNet
- National Institute for Health Research
- University College London Hospitals Biomedical Research Centre
- CRUK UCL Experimental Cancer Medicine Centre
- Wellcome Trust Senior Fellow in Clinical Science [WT107963/Z/15/Z]
- Francis Crick Institute [FC001169, FC001202]
- UK Medical Research Council [FC001169, FC001202]
- MRC [G108/596] Funding Source: UKRI
Ask authors/readers for more resources
Pre-clinical non-small cell lung cancer (NSCLC) models are poorly representative of the considerable inter-and intra-tumor heterogeneity of the disease in patients. Primary cell-based in vitro models of NSCLC are therefore desirable for novel therapy development and personalized cancer medicine. Methods have been described to generate rapidly proliferating epithelial cell cultures from multiple human epithelia using 3T3-J2 feeder cell culture in the presence of Y-27632, a RHO-associated protein kinase (ROCK) inhibitor, in what are known as conditional reprograming conditions (CRC) or 3T3 + Y. In some cancer studies, variations of this methodology have allowed primary tumor cell expansion across a number of cancer types but other studies have demonstrated the preferential expansion of normal epithelial cells from tumors in such conditions. Here, we report our experience regarding the derivation of primary NSCLC cell cultures from 12 lung adenocarcinoma patients enrolled in the Tracking Cancer Evolution through Therapy (TRACERx) clinical study and discuss these in the context of improving the success rate for in vitro cultivation of cells from NSCLC tumors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available