Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 118, Issue -, Pages 2040-2050Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.07.075
Keywords
Saccharide; Magnetic cross-linked enzyme aggregates; Maltose and sucrose
Funding
- Scientific Research Projects Commission of Sakarya University [2017-02-04-050]
- Turkish Academy of Sciences (TUBA)
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The cross-linked enzyme aggregates (CLEAs) involves formation of a number of covalent bonds between enzyme and the matrix using glutaraldehyde. In general, amino groups of lysine, sulthydryl groups of cysteine, phenolic OH groups of tyrosine, or imidazol group of histidine are used for enzyme binding under mild conditions. The main advantage of this method is its simplicity, economic advantages in the industrial bio catalysis. The Fe3O4 magnetic nanoparticles were synthesized by coprecipitating Fe2+ and Fe3+ in alkaline solution. Tannic acid was used to functionalize the Fe3O4 magnetic nanoparticles. After functionalization process, tannic acid magnetic cross-linked enzyme aggregates of enzyme (TA-MNPs-CLEAs) were prepared by cross-linking of enzyme aggregates with different saccharides as additive. The present result reported high stability, simplicity, low cost and recyclability of a saccharide-TA-MNPs-CLEAs-enzyme make it efficient as a highly active biocatalyst in biotechnological applications. The obtained results suggest that disaccharides (maltose, sucrose and lactose) and polysaccharide such as starch are eco-friendly additives to TA-MNPs-lipase and TA-MNPs-CLEAs-peroxidase and can become a powerful biocatalyst in industry applications. (C) 2018 Elsevier B.V. All rights reserved.
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