Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 107, Issue -, Pages 1320-1329Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.09.116
Keywords
Marine alga; Lectin; Anticancer effect
Funding
- Brazilian agencies CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- FUNCAP (Fundacao Cearense de Apoio ao Desenvolvimento Cientlfico e Tecnologico)
- FINEP (Financiadora de Estudos e Projetos)
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As described in the literature, Solieria filiformis lectin (SfL) from the marine red alga S. filiformis was found to have antinociceptive and anti-inflammatory effects. In this study, we characterized two SfL variants, SfL-1 and SfL-2, with molecular mass of 27,552 Da and 27,985 Da, respectively. The primary structures of SfL-1 and SfL-2 consist of four tandem-repeat protein domains with 67 amino acids each. SfL-1 and -2 showed high similarity to OAAH-family lectins. 3D structure prediction revealed that SfL-1 and -2 are composed of two beta-barrel-like domains formed by five antiparallel beta-strands, which are connected by a short peptide linker. Furthermore, the mixture of isoforms (SfLs) showed anticancer effect against MCF-7 cells. Specifically, SfLs inhibited 50% of viability in MCF-7 cells after treatment at 125 mu g.mL(-1), while the inhibition of Human Dermal Fibroblasts (HDF) was 34% with the same treatment. Finally, 24 h after treatment, 25% of MCF-7 cells were in early apoptosis and 35% in late apoptosis. Evaluation of pro- and anti-apoptotic gene expression of MCF-7 cells revealed that SfLs induced caspase-dependent apoptosis within 24 h. (C) 2017 Elsevier B.V. All rights reserved
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