Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 107, Issue -, Pages 755-761Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.09.055
Keywords
Mannan from yeast cell walls; Derivatives; Antioxidant activities
Funding
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
- Chongqing Key Research Project of Basic Science & Frontier Technology [cstc2017jcyjBX0012]
- Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJ1400523]
- Foundation Project of Chongqing Normal University [14XYY020]
- Chongqing General Research Program of Basic Research and Frontier Technology [cstc2015jcyjA10054]
- Chongqing Normal University Postgraduate's Research and Innovation Project [YKC17004]
- Open Foundation Project of Engineering Research Center for Bioactive Substances [AS201614]
- Chongqing University Students' Training Project of Innovation & Undertaking, China [201610637076]
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The alkaline mannan (M) was extracted from yeast cell walls, and the best extraction condition was: 100 degrees C, 2 h, 1 % NaOH, solid-liquid ratio of 10:1. The purity of mannan was 96.15%. Its five derivatives were obtained by chemical modification methods, and they were: phosphorylated mannan (P-M), sulfated mannan (S-M), carboxymethylated mannan (CM-M), carboxymethylated-phosphorylated mannan (CMP- M), and carboxymethylated-sulfated mannan (CMS-M). The phosphate substitution degree of P-M and CMP-M was 0.2 and 0.1, respectively. The sulfate substitution degree of S-M and CMS-M was 0.67 and 0.65, respectively. The carboxymethyl substitution degree of CM-M, CMP-M and CMS-M were 1.11,1.17 and 0.25, respectively. It indicated that the hydroxyl radical scavenging capacities of P-M and CMP-M were 15% higher than that of M. The anti-lipid peroxidation capacity of all derivatives increased than that of M, especially for CMP-M, its scavenging effect was 25% higher. (C) 2017 Elsevier B.V. All rights reserved.
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