Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 109, Issue -, Pages 837-846Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.11.053
Keywords
Copper-chelating; Iron-chelating; Mitogenic activity; Cell wall polysaccharide; Nitric oxide
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnoleigico-CNPq
- Coordenacao de Aperfeicoamento Pessoal de Nivel Superior-CAPES
- Ministry of Science, Technology and Information - MCTIC
- CNPq
- CAPES
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This study evaluated the antioxidant, antiproliferative, and immunostimulatory properties of cell wall alpha-D-mannan fractions from yeast Kluyveromyces marxianus CCT7735. Filter centrifugation was used to obtain four fractions (KMM-1, KMM-2, KMM-3, and KMM-4) with molecular weight ranging from 7.6 to 75.1 kDa. KMM-1 and KMM-2 comprised D-mannose with traces of D-glucose, whereas other fractions contained only D-mannose. Total sugar found in samples ranged from 85.9% to 96.1%, while protein and phenolic contents were 1.21% and 0.41%, respectively. Although only KMM-1 was able to scavenge superoxide radicals, all fractions presented total antioxidant capacity as well as reducing power, hydroxyl-radical scavenging, and copper- and iron-chelating activities. No fraction was cytotoxic to HeLa cells. However, all samples inhibited the proliferation of the tumor cell Hep-G2 and presented minor cytotoxicity against normal 3T3 cells. All fractions showed mitogenic activity in macrophages and all, except KMM-4, induced nitric oxide production in macrophages, suggestive of their immunostimulatory effects. (C) 2017 Elsevier B.V. All rights reserved.
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