Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 108, Issue -, Pages 56-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.11.148
Keywords
Bacillus mojavensis; Alkaline proteases; alpha-Amylases; Detergent stable; Dehairing function
Funding
- Ministry of Higher Education and Scientific Research, Tunisia
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The present work aims to study the simultaneous production of highly alkaline proteases and thermostable alpha-amylases by a newly isolated bacterium Bacillus mojavensis SA. The optimum pH and temperature of amylase activity were 9.0 and 55 degrees C, respectively, while those of the proteolytic activity were 12.0 and 60 degrees C, respectively. Both alpha-amylase and protease enzymes showed a high stability towards a wide range of pH and temperature. Furthermore, SA crude enzymes were relatively stable towards non-ionic (Tween 20, Tween 80 and Triton X-100) and anionic (SDS) surfactants, as well as oxidizing agents. Both activities were improved by the presence of polyethylene glycol 4000 and glycerol. Additionally, the crude enzymes showed excellent stability against various solid and liquid detergents. Wash performance analysis revealed that the SA crude enzymes exhibited a remarkable efficiency in the removal of a variety type of stains, such as blood, chocolate, coffee and oil. On the other side, SA proteases revealed a potential dehairing activity of animal hide without chemical assistance or fibrous proteins hydrolysis. Thus, considering their promising properties, B. mojavensis SA crude enzymes could be used in several biotechnological bioprocesses. (C) 2017 Elsevier B.V. All rights reserved.
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