Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 109, Issue -, Pages 740-747Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.11.065
Keywords
Allicin; Nanoprecipitation; Locust bean gum; Therapeutics; In silico analysis; Drug delivery
Funding
- University Grants Commission (UGC)
- Dr. D.S.Kothari Post Doctoral Fellowship, New Delhi [F.4-2/2006(BSR)/BL/14-15/0480]
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The field of nanotechnology has overgrown over the past few years and has even ventured into the field of medicine. The aim of the present study is to develop a novel allicin functionalized locust bean gum nanoparticle using the nanoprecipitation technique. The synthesized nanoparticles were characterized by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The characterization study revealed the nanoscale structure (similar to 100 nm) of the prepared particles. In silico toxicology analysis were carried out to assess the drug-like properties and virtual toxicity of allicin. Toxicity of the prepared nanoparticles were carried out in RAW 264.7 cell lines in vitro and in vivo studies were carried out in Sprague-Dawley rats. In in vitro study, LBGAN showed a maximum toxicity of 10.51% in MU assay, no reactive oxygen species generation on DCFDA staining and LBGAN was effective to protect the cells from apoptosis. In in vivo toxicity studies LBGAN showed no significant change in the activities of the marker enzymes like LDH, CK-MB, ALP, ACP, AST and ALT. Thus, the functionalization of nanoparticles with allicin has the benefit of providing protection and stability to the allicin, in addition to increasing its pharmacological activity. (C) 2017 Elsevier B.V. All rights reserved.
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