Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 108, Issue -, Pages 376-382Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.11.128
Keywords
Oxidized konjac glucomannan; Chitosan-collagen peptide; Hydrogel; Blood clotting activity; Wound healing
Funding
- National Natural Science Foundation of China [51773161]
- Special Funds Project of Major New Products of Hubei Province [20132h0040]
- University-industry Cooperation Projects of The Ministry of Education of Guangdong province [2012B091100437]
- innovation fund project of the Ministry of Science and Technology of Small and Medium-sized Enterprises [11C26214202642, 11C26214212743]
- Zhuhai Science and Technology Plan Projects [2011B050102003]
- Wuhan Science and Technology Development [201060623262]
- Fundamental Research Funds for the Central Universities [2014-zy-220]
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In this paper, the microbial transglutaminase (MTGase) was used as a catalyst to graft the collagen peptide (COP) molecules on the amino group of chitosan to obtain water-soluble chitosan-collagen peptide (CS-COP) derivatives. The preparation of composite hydrogel was via the Schiff-base reaction between the amino of CS-COP and the aldehyde of oxidized konjac glucomannan (OKGM). The hydrogels were characterized by various techniques including Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The results of SEM showed that the hydrogel sample had a clear and stable three-dimensional network structure. Meanwhile, these effects of the addition of OKGM on gelation time, swelling behaviors, water evaporation rate and blood coagulation capacity were investigated. The shortest gelation time for hydrogels was 99.3 s. The hydrogels showed a good swelling ability and appropriate water retention capacity. The maximum swelling ratio of the hydrogel was 265%. Dynamic blood clotting test showed that the hydrogels materials had good blood coagulation capacity. Moreover, The biocompatibility of hydrogels was evaluated with NIH-3T3 cells by MIT method. The results indicated that the hydrogels exhibited better biocompatibility. Therefore, this hydrogel has a promising potential to be applied as wound dressing. (C) 2017 Published by Elsevier B.V.
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