Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 108, Issue -, Pages 47-55Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.11.111
Keywords
Lycium barbarum; Polysaccharide; Anti-angiogenesis; Sulfated derivative
Funding
- National Natural Science Foundation of China [31230022]
- New Drug Creation and Manufacturing Program [2017ZX09305-003]
- Ministry of Science and Technology, the People Republic of China
- Program of Shanghai Subject Chief Scientist [16XD1404500]
- Chinese Academy of Sciences
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Angiogenesis plays a crucial role in tumor growth and development. Blocking angiogenesis of tumor cells has become one of the most promising approaches in cancer therapy. Here, an acidic polysaccharide designated LBP1B-S-2 with an average molecular weight of 80.00 kDa, was extracted and purified from dried mature fruits of Lycium barbarum L by DEAE Sepharose (TM) Fast Flow and Sephacryl S-300 HR columns. Monosaccharide composition analysis indicated that the LBP1B-S-2 was composed of rhamnose, arabinose, galactose and glucuronic acid in a molar ratio of 3.13: 53.55: 39.37: 3.95. The backbone of LBP1B-S-2 was consisted of 1, 3-linked beta-D-Galp, 1, 6-linked beta-D-Galp and branches contained 1, 4-linked beta-D-GlcpA, T-linked beta-D-Galp, 1, 6-linked beta-D-Galp, T-linked alpha-L-Araf, T-linked beta-L-Aral 1, 5-linked alpha-L-Araf and T-linked beta-L-Rhap directly or indirectly attached to C-3 position of 1, 6-linked beta-D-Galp or C-6 position of 1, 3-linked beta-D-Galp, according to the results of partial acid hydrolysis analysis, methylation analysis, IR and NMR spectra. The sulfated derivative of LBP1B-S-2, could significantly inhibit the tube formation of human microvascular endothelial cells (HMEC-1) in vitro at concentration of (95 nM) without significant cytotoxicity. (C) 2017 Published by Elsevier B.V.
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