Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 115, Issue -, Pages 978-984Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.04.145
Keywords
Exopolysaccharides; A. auricula-judae; Structural characterization; lmmunomodulatory activity
Funding
- National High Technology and Research Development Program of China [2013AA102105]
- Science and Technology Project of Tianjin [16YFXTNC00160]
- Tianjin Enterprise Postdoctoral Innovation Project
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Submerged culture of Auricularia auricula-judae has been documented, but there have been few studies on the structural characterization and medicinal properties of its exopolysaccharides. In present study, two exopolysaccharides, named CEPSN-1 and CEPSN-2, were isolated from submerged culture of A. auricula-judae, and their structural features as well as immunomodulatory activity were analyzed. The two exopolysaccharides both had a backbone chain composed of (1 -> 4)-alpha-D-glucose residues in glucopyranose type. At the tested concentration range of 50-200 mu g/mL, CEPSN-1 and CEPSN-2 not only showed non-toxicity to RAW 264.7 cells, but also could promote the release of NO and cytokines (IL-6, IL-10 and TNF-alpha) in the cells. The release of NO was significantly enhanced by the two exopolysaccharides at 100 mu g/mL (p < 0.05). The IL-10 secretion was significantly increased by 1.80 and 1.61-fold, compared to the control after treatment with 50 mu g/mL of CEPSN-1 and CEPSN-2, respectively (p < 0.05). These results demonstrated that, though their structural feature were different from that of polysaccharides from fruit body, exopolysaccharides of A. auricula-judae from submerged culture with the backbone of (1 -> 4)-alpha-D-glucan could be explored as potential immunomodulatory agents for the application in complementary medicine or functional foods. (C) 2018 Elsevier B.V. All rights reserved.
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