Journal
JOURNAL OF PLANT PATHOLOGY
Volume 96, Issue 2, Pages 281-285Publisher
EDIZIONI ETS
Keywords
coconut; diagnosis; nested PCR; Ghana; phytoplasma
Categories
Ask authors/readers for more resources
Lethal Yellowing-type diseases of coconut have destroyed thousands of coconut palms globally, and in Ghana the disease [Cape St. Paul wilt disease (CSPWD)] is considered as the single most important factor affecting the coconut industry. To improve the diagnostics available for CSPW detection, a ribosomal and a non-ribosomal-based assay were assessed for their effectiveness in diagnosing CSPWD. PCR diagnoses and observation of symptom development were carried out twice per year over three years to study the fate of infected palms and the pattern of disease spread. Comparison of the effectiveness of different PCR assays at detecting CSPWD-infection showed a comparable performance of a single round secA-based assay to nested PCR using primers targeting ribosomal DNA sequences. The study showed that all infected palms eventually died from infection and analysis of the location of palms becoming infected showed no clear pattern in the spread of infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available