Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 52, Issue 1, Pages 35-41Publisher
ELSEVIER
DOI: 10.1016/j.ijantimicag.2018.03.002
Keywords
Pseudomonas aeruginosa; Quorum sensing; Biofilm; Aminoglycoside antibiotics; Antibiotic accelerant
Funding
- National Key Research and Development Program of China [2017YFD0201401]
- Natural Science Foundation of Jiangsu Province, China [BK20170859]
- Science and Technology Development Program of Modern Agriculture, Nanjing [201608052]
- Six Talent Peaks Project in Jiangsu Province
- Fundamental Research Funds for the Central Universities [30916011307]
- National Natural Science Foundation of China [31170131]
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Pseudomonas aeruginosa infection is a serious disease in cystic fibrosis patients and is difficult to treat owing to biofilm persistence and emerging multidrug resistance. Considering the essential role of quorum sensing (QS) in P. aeruginosa infections, the enhanced effects between the quorum sensing inhibitor (QSI) resveratrol and several antibiotics against P. aeruginosa PAO1 were investigated. Crystal violet staining assay revealed that biofilms of P. aeruginosa PAO1 grown in the presence of resveratrol were more susceptible to aminoglycoside antibiotics. Scanning electron and fluorescence microscopy showed architectural disruption of the biofilms when treated with resveratrol and aminoglycoside antibiotics. Furthermore, quantitative reverse transcription PCR (qRT-PCR) analysis demonstrated that expression of lasI and rhlI, two genes encoding enzymes that synthesise signal molecules in QS systems, were inhibited in the P. aeruginosa PAO1 biofilms by resveratrol. These results indicate that the QSI resveratrol can significantly enhance the effects of aminoglycoside antibiotics (e.g. tobramycin, gentamicin, amikacin and netilmicin) on P. aeruginosa PAO1 biofilms. These findings suggest that resveratrol is a potential accelerant in the treatment of P. aeruginosa biofilms and can restore or enhance the efficacy of aminoglycoside antibiotics. (c) 2018 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
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