Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 60, Issue -, Pages 202-210Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2018.05.004
Keywords
Fisetin; Anti-inflammation; ICAM-1; COX-2; ERK1/2; NF-kappa B
Categories
Funding
- Chang Gung Memorial Hospital, Linkou [CMRPF1G0231, CMRPF1F0082, CMRPF1G0181, CMRPG3C0901]
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Fisetin, a flavone that can be isolated from fruits and vegetables, has anti-tumor and anti-oxidative properties and ameliorates airway hyperresponsiveness in asthmatic mice. This study investigated whether fisetin can suppress the expression of inflammatory mediators and intercellular adhesion molecule 1 (ICAM-1) in A549 human lung epithelial cells that were stimulated with interleukin-1 beta (IL-1 beta) to induce inflammatory responses. A549 cells were treated with fisetin (3-30 mu M) and then with IL-1 beta. Fisetin significantly inhibited COX-2 expression and reduced prostaglandin E-2 production, and it suppressed the levels of IL-8, CCL5, monocyte chemotactic protein 1, tumor necrosis factor alpha, and IL-6. Fisetin also significantly attenuated the expression of chemokine and inflammatory cytokine genes and decreased the expression of ICAM-1, which mediates THP-1 monocyte adhesion to inflammatory AS49 cells. Fisetin decreased the translocation of nuclear transcription factor kappa-B (NF-kappa B) subunit p65 into the nucleus and inhibited the phosphorylation of proteins in the ERK1/2 pathway. Co-treatment of IL-1 beta-stimulated A549 cells with ERK1/2 inhibitors plus fisetin reduced ICAM-1 expression. Furthermore, fisetin significantly increased the effects of the protective antioxidant pathway by promoting the expression of nuclear factor erythroid-2-related factor-2 and heme oxygenase 1. Taken together, these data suggest that fisetin has anti-inflammatory effects and that it suppresses the expression of chemokines, inflammatory cytokines, and ICAM-1 by suppressing the NF-kappa B and ERK1/2 signaling pathways in IL-1 beta-stimulated human lung epithelial A549 cells.
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