Removal of gaseous dichloromethane using a solid-liquid partitioning bioreactor under gradual and stepped load increase

Silicone rubber was used as the solid non-aqueous phase of two-phase partitioning bioreactor (TPPB) for dichloromethane (DCM) removal. When the load gradually increased from 150 g m(-3) h(-1)-485 g m(-3) h(-1) by increasing the gaseous DCM concentration, a steady elimination capacity (EC) of approximately 130 g m(-3) h(-1) was achieved in one-liquid-phase completely mixed stirred tank bioreactor (CSTB), whereas the EC of TPPB was enhanced from 155 g m(-3) h(-1)-366 g m(-3) h(-1). This result indicated that DCM removal was controlled by the biological process in CSTB and by mass transfer in TPPB. Meanwhile, the biomass decreased from 534 mg l(-1) to 381 mg l(-1) in CSTB compared with that maintained at around 580 mg l(-1) in TPPB due to the different resistance capabilities to DCM toxicity. Dehalogenase activity was maintained at a relatively high level under high DCM stress with the addition of silicon rubber. TPPB was shown to be more dependable in the overload from 120 g m(-3) h(-1)-450 g m(-3) h(-1). This paper is the first to report that the crucial step in high-load pollutant removal can be converted from the biological process to mass transfer by adding solid non-aqueous phase.