Journal
INTEGRATIVE CANCER THERAPIES
Volume 17, Issue 4, Pages 1204-1215Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1534735418786027
Keywords
pancreatic cancer; Pao Pereira; cancer stem-like cells; xenograft models
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Funding
- Beljanski Foundation
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Pancreatic cancers are enriched with cancer stem-like cells (CSCs), which are resistant to chemotherapies, and responsible for tumor metastasis and recurrence. Here, we investigated the extract of a medicinal plant Pao Pereira (Pao) for its activity against pancreatic CSCs. Pao inhibited overall proliferation of human pancreatic cancer cell lines with IC50 ranging from 125 to 325 mu g/mL and had limited cytotoxicity to normal epithelial cells. Pancreatic CSC population, identified using surface markers CD24+ CD44+ EpCam+ or tumor spheroid formation assay, was significantly reduced, with IC(50)s of similar to 100 mu g/mL for 48 hours treatment, and similar to 27 mu g/mL for long-term treatment. Nuclear beta-catenin levels were decreased, suggesting suppression of Wnt/beta-catenin signaling pathway. In vivo, Pao at 20 mg/kg, 5 times/week gavage, significantly reduced tumorigenicity of PANC-1 cells in immunocompromised mice, indicating inhibition of CSCs in vivo. Further investigation is warranted in using Pao as a novel treatment targeting pancreatic CSCs.
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