Journal
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 100, Issue -, Pages 66-77Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2018.06.006
Keywords
Cytochrome P450; Exogenous chemical; Gene expression; Locusta migratoria; Transcriptome
Categories
Funding
- National Natural Science Foundation of China [31320103921, 31402020]
- Shanxi Province Science Foundation [201601D011073]
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Cytochrome P450 monooxygenases (CYPs) belong to a large superfamily of heme-containing enzymes catalyzing at least 60 different types of chemically distinct reactions. Insect CYPs play key roles in biotransformation of insecticides and plant chemicals, and are implicated in insecticide resistance and insect adaptation to their host plants. Insect CYPs are well studied in model insects, but little is known about the CYP superfamily in paurometabolous insects. We employed Illumina sequencing technology to identify 71 partial and 78 full-length open reading frames (ORFs) of LmCYP genes from the migratory locust (Locusta migratoria), one of the most destructive paurometabolous insect pests in the world. Seventy-eight LmCYPs with complete ORFs were formally named and classified into 19 families and 43 subfamilies. The majority of LmCYPs were mainly expressed in nymphal and adult stages, but LmCYP expression varied widely among thirteen different tissues examined. Regulatory elements were predicted in the promoter regions of LmCYP genes, and subsequent exposure of locusts to 12 different exogenous chemicals showed that 2-tridecanone and xanthotoxin were the most effective at increasing LmCYP expression. Our results represent the first transcriptome-wide analysis of the LmCYP super family from migratory locust, and provide a foundation for understanding the physiological functions, functional diversity, evolution, and regulatory mechanisms controlling the expression of the CYP gene superfamily in the locust.
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