Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 16, Pages 8923-8931Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b02156
Keywords
switch; metal ion; responsive; acrylamide; polymer
Funding
- EU FET Open MICRE-Agents Project [318671]
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Acrylamide/acrylamide-modified nucleic acid copolymer chains provide building units for the construction of acrylamide-DNA hydrogels. Three different hydrogels are prepared by the cross-linking of the acrylamide-DNA chains with metal ion-dependent DNAzyme sequences and their substrates. The metal ion-dependent DNAzyme sequences used in the study include the Cu2+-, Mg2+-, and Zn2+-dependent DNAzymes. In the presence of the respective metal ions, the substrates of the respective DNAzymes are cleaved, leading to the separation of the cross-linking units and to the dissolution of the hydrogel. The different hydrogels were loaded with a fluorophore-modified dextran or with a fluorophore-functionalized glucose oxidase. Treatment of the different hydrogels with the respective ions led to the release of the loaded dextran or the enzyme, and the rates of releasing of the loaded macromolecules followed the order of Cu2+ > Mg2+ > Zn2+. Also, the different hydrogels were loaded with the enzymes beta-galactosidase (beta-Gal), glucose oxidase (GOx), or horseradish peroxidase (HRP). In the presence of the appropriate metal ions, the respective hydrogels were dissolved, resulting in the activation of the B-Gal/GOx or GOx/HRP bienzyme cascades and of the beta-Gal/GOx/HRP trienzyme cascade.
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