Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 29, Pages 16011-16017Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b04295
Keywords
boronic-acid-functionalized magnetic graphene; enrichment; glycoproteome; phenolic-formaldehyde resin; MALDI-TOF mass spectrometry
Funding
- National Basic Research Program of China [2012CB910604]
- National High-Tech RD Program [2012AA020202]
- National Natural Science Foundation of China [21275034, 21475027]
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Capturing glycopeptides selectively and efficiently from mixed biological samples has always been critical for comprehensive and in-depth glycoproteomics analysis, but the lack of materials with superior capture capacity and high specificity still makes it a challenge. In this work, we introduce a way first to synthesize a novel boronic-acid-functionalized magnetic graphene@phenolic-formaldehyde resin multilayer composites via a facile process. The as-prepared composites gathered excellent characters of large specific surface area and strong magnetic responsiveness of magnetic graphene, biocompatibility of resin, and enhanced affinity properties of boronic acid. Furthermore, the functional graphene composites were shown to have low detection limit (1 fmol) and good selectivity, even when the background nonglycopeptides has a concentration 100 fold higher. Additionally, enrichment efficiency of the composites was still retained after being used repeatedly (at least three times). Better yet, the practical applicability of this approach was evaluated by the enrichment of human serum with a low sample volume Of 1 mu L. All the results have illustrated that the magG@PF@APB has a great potential in glycoproteome analysis of complex biological samples.
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