Journal
INFLAMMATION
Volume 41, Issue 5, Pages 1780-1790Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-018-0821-8
Keywords
sepsis; secondary infection; nosocomial infection; sepsis; regulatory T cells; CD25
Categories
Funding
- National Natural Science Foundation of China [81401621] Funding Source: Medline
- Natural Science Foundation of Zhejiang Province [LY13H150004] Funding Source: Medline
- Excellent Young Talents Program of Traditional Chinese Medicine of Zhejiang Province [2013ZQ023] Funding Source: Medline
- Wenzhou Municipal Science and Technology Project [2015KYB239] Funding Source: Medline
Ask authors/readers for more resources
Immune dysfunction contributes to secondary infection and worse outcomes in sepsis. Regulatory T cells (Tregs) have been implicated in sepsis-induced immunosuppression. Nevertheless, the role of Tregs in secondary infection after sepsis remains to be determined. In the present study, a two-hit model which mimics clinical conditions was used and the potential role of Tregs in secondary Pseudomonas aeruginosa infection post-sepsis was investigated. Results showed that mice were susceptible to secondary P. aeruginosa infection 3days, but not 7days, post-cecal ligation and puncture (CLP). The levels of IL-17A, IL-1, and IL-6 remained low in CLP mice after P. aeruginosa infection, while the levels of IL-10 increased significantly. Additionally, increased number of Tregs in both lung and spleen was observed in two-hit mice. Injection with PC61 (anti-CD25) mAb reduced the number of Tregs by 50% in spleen and 60% in lung of septic mice. This partial depletion of Tregs elevated IL-17A, IL-1, and IL-6 production and decreased IL-10 levels in septic mice with P. aeruginosa infection, leading to lower bacterial load, attenuation of lung injury, and improvement of survival. The present findings demonstrate that Tregs play a crucial role in secondary P. aeruginosa infection after sepsis by modulating the inflammatory response.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available