Journal
INFECTION AND IMMUNITY
Volume 86, Issue 9, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00244-18
Keywords
Toxoplasma gondii; host-pathogen interactions; mTOR; macrophages; translational control
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Funding
- Basil O'Connor starter scholar research award [5-FY14-78]
- March of Dimes Foundation [6-FY16-151]
- Subvention de Regroupement Strategique from the Fonds de Recherche du Quebec en Nature et Technologies (FRQ-NT)
- Bourse de Chercheur-Boursier Junior 1 from the Fonds de Recherche du Quebec en Sante (FRQ-S)
- Subvention d'Etablissement de Jeune Chercheur from the FRQ-S
- Fondation Universitaire Armand Frappier
- Swedish Research Council
- Wallenberg Academy Fellows program
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The intracellular parasite Toxoplasma gondii promotes infection by targeting multiple host cell processes; however, whether it modulates mRNA translation is currently unknown. Here, we show that infection of primary murine macrophages with type I or II T. gondii strains causes a profound perturbation of the host cell translatome. Notably, translation of transcripts encoding proteins involved in metabolic activity and components of the translation machinery was activated upon infection. In contrast, the translational efficiency of mRNAs related to immune cell activation and cytoskeleton/cytoplasm organization was largely suppressed. Mechanistically, T. gondii bolstered mechanistic target of rapamycin (mTOR) signaling to selectively activate the translation of mTOR-sensitive mRNAs, including those with a 5'-terminal oligopyrimidine (5' TOP) motif and those encoding mitochondrion-related proteins. Consistent with parasite modulation of host mTOR-sensitive translation to promote infection, inhibition of mTOR activity suppressed T. gondii replication. Thus, selective reprogramming of host mRNA translation represents an important subversion strategy during T. gondii infection.
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