Journal
IET NANOBIOTECHNOLOGY
Volume 12, Issue 4, Pages 509-513Publisher
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2017.0105
Keywords
antibacterial activity; catalysis; nanoparticles; gold; nanofabrication; biomedical materials; nanomedicine; renewable materials; surface diffusion; dyes; water pollution; reaction rate constants; toxicology; antibacterial activity; catalytic activity; biogenic gold nanoparticles; Trichoderma harzianum; biomass; growth curve; diffusion; colony forming unit count methods; minimum inhibitory concentration; optical absorption; CFU methods; methylene blue; water pollutant; catalytic degradation; toxic pollutants; sodium borohydrate; rate constant; Au
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This study reveals the antibacterial and catalytic activity of biogenic gold nanoparicles (AuNPs) synthesised by biomass of Trichoderma harzianum. The antibacterial activity of AuNPs was analysed by the means of growth curve, well diffusion and colony forming unit (CFU) count methods. The minimum inhibitory concentration of AuNPs was 20 mu g/ml. AuNPs at 60 mu g/ml show effective antibacterial activity as optical absorption was insignificant. The well diffusion and CFU methods were also applied to analyse the effect of various concentration of AuNPs. Further, the catalytic activity of AuNPs was analysed against methylene blue (MB) as a model pollutant in water. MB was degraded 39% in 30 min in the presence of AuNPs and sodium borohydrate and the rate constant (k) was found to be 0.2 x 10(-3) s(-1). This shows that the biogenic AuNP is an effective candidate for antibacterial and catalytic degradation of toxic pollutants.
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