Journal
IET NANOBIOTECHNOLOGY
Volume 12, Issue 2, Pages 138-146Publisher
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2017.0029
Keywords
nanocomposites; iron compounds; calcium compounds; cerium; mechanical strength; antibacterial activity; biomedical materials; dentistry; bone; nanoparticles; nanofabrication; ball milling; Fourier transform infrared spectra; attenuated total reflection; X-ray diffraction; magnetometry; scanning electron microscopy; transmission electron microscopy; microorganisms; cellular biophysics; nanomedicine; Ce-doped HAP-Fe3O4 nanocomposite; hydroxyapatite; in vitro biological evaluation; mechanical strength; bacterial restriction behaviour; metal ion; silver ion; zinc ion; copper ion; titanium ion; cerium ion; HAP matrix; antibacterial agent; thermal stability; dental filling; bone healing; catheter; Fe3O4 nanoparticle; hyperthermia treatment; magnetic fluid recording; catalysis; ball milling technique; Fourier transform infrared spectroscopy; attenuated total reflectance spectroscopy; X-ray diffraction; vibrating sample magnetometry; scanning electron microscopy; SEM-energy dispersive spectroscopy; transmission electron microscopy; TEM analysis; antibacterial activity; Staphylococcus aureus; Escherichia coli; pathogen inhibition; in vitro biocompatibility; MG-63 osteoblast cell; cell enhancement; Ca-5(PO4)(3)(OH):Ce; Fe3O4
Funding
- DST, New Delhi, India [SB/FT/CS-091/2012]
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Hydroxyapatite (HAP: Ca-10(PO4)(6)(OH)(2)) is extensively used in biomedical field because of its biocompatibility, osteoconductivity and non-toxicity properties. However, HAP exhibits poor mechanical strength and bacterial restriction behavior. To overcome these drawbacks, various metal ions such as Ag+, Zn2+, Cu2+, Ti4+ and Ce4+/3+ are incorporated in HAP matrix to increase the mechanical and biological properties. Among these, Cerium (Ce) is selected as antibacterial agent due to its high thermal stability and its applications in dental fillings, bone healing and catheters. Fe3O4 nanoparticles were used in hyperthermia treatment, magnetic fluid recordings and catalysis. In this present study, we have synthesized nanocomposites consisting of 1.25% Ce doped HAP with various concentrations of Fe3O4 NPs as 90:10 (C-1), 70:30 (C-2) and 50:50 wt% (C-3) using ball milling technique. The obtained Ce@HAP-Fe3O4 nanocomposites were characterized by ATR-FTIR, XRD, VSM, SEM-EDAX and TEM analysis. Further, the fabricated Ce@HAP-Fe3O4 nanocomposites were tested for its antibacterial activity towards Staphylococcus aureus (S. aureus) and Escherichia coli (E.coli), where C-3 composites exhibit the excellent pathogen inhibition towards E.coli. In addition, the cytotoxicity evaluation on C-3 nanocomposites by in vitro biocompatibility study using MG-63 cells shows the prominent viable cell enhancement up to 400 mu g/mL concentrations.
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