Journal
AGROBACTERIUM PROTOCOLS, VOL 1, 3RD EDITION
Volume 1223, Issue -, Pages 43-56Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-4939-1695-5_4
Keywords
In vitro culture; Legumes; Medicago; Transgenic plants
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Legumes have been for a long time recalcitrant to efficient Agrobacterium transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on Agrobacterium-mediated transformation of M. truncatula followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for M. truncatula R108-1 (c3).
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