Journal
APOPTOSIS AND CANCER: METHODS AND PROTOCOLS, 2ND EDITION
Volume 1219, Issue -, Pages 1-9Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-4939-1661-0_1
Keywords
Caspase-3; Apoptosis; Irradiation; PARP-1; Flow cytometry; Immunohistochemistry
Funding
- NCI NIH HHS [CA127264] Funding Source: Medline
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Apoptosis can be measured by number of methods by taking advantage of the morphological, biochemical, and molecular changes undergoing in a cell during this process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fluorochrome substrates, as well as employing the fluorescein-labeled CaspaTag pan-caspase in situ detection kit.
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