Journal
DESIGNED MONOMERS AND POLYMERS
Volume 18, Issue 2, Pages 129-136Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/15685551.2014.971392
Keywords
protein adsorption; click chemistry; bioconjugation; photopolymerization; hydrogels
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Funding
- Istanbul Technical University Research Fund
- TUBITAK (The Scientific and Technical Research Council of Turkey) [113Z234]
- TUBITAK
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A simple approach for the preparation of a clickable poly(ethylene glycol)-based hydrogel as a polymeric support for protein immobilization via photoinitiated free radical polymerization of poly(ethylene glycol) diacrylate and propargyl acrylate is established. Bioconjugation to the obtained gels was achieved by azide-alkyne click reaction with azido-functionalized anti-immunoglobulin G (anti-IgG) and anti-His tag antibodies. Evaluation of the affinities of the PEG hydrogels to the corresponding substrates (IgG and His-tagged YFP, respectively) indicates their specific binding capability.
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