Detection of Cell Morphological Changes of Ischemic Rabbit Liver Tissue Using Bioimpedance Spectroscopy

Bioimpedance spectroscopy is a promising method for tissue ischemia monitoring; it is used in this research to study ischemia and cell morphology using bioimpedance measurements of rabbit liver tissue. This paper presents the evolution of Cole parameters and cell parameters with time and establishes a relation between cellular morphological changes during cell ischemia with bioimpedance measurements at different frequencies. The general behavior of ischemic liver tissue has been analyzed. Evolution of Cole parameters was extrapolated from BIS measurements, and their behavior was studied with respect to time. Then, cell parameters were estimated from Cole parameters. A detailed analysis of the changes of cell parameters was done in parallel with explanations for these changes depending on the physiological mechanism of ischemia and necrosis bioprocess. These explanations give a confirmation of the established relation between cell morphological changes and the corresponding BIS measurements.