Journal
EUROPEAN JOURNAL OF ENDOCRINOLOGY
Volume 172, Issue 1, Pages -Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/EJE-14-0492
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Funding
- Health and Performance Enhancement Division of Pennington Biomedical Research Center
- Nutrition Obesity Research Center (NORC) [NIH 1P30 DK072476]
- COBRE [NIH P20 GM103528]
- NORC [NIH 1P30-DK072476]
- NIH [R00HD060762, U01DK094418]
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Objective: Polycystic ovary syndrome (PCOS) is associated with reduced adipose tissue lipolysis that can be rescued by aerobic exercise. We aimed to identify differences in the gene expression of perilipins and associated targets in adipose tissue in women with PCOS before and after exercise. Design and methods: We conducted a cross-sectional study in eight women with PCOS and eight women matched for BMI and age with normal cycles. Women with PCOS also completed a 16-week prospective aerobic exercise-training study. Abdominal subcutaneous adipose tissue biopsies were collected, and primary adipose-derived stromal/stem cell cultures were established from women with PCOS before 16 weeks of aerobic exercise training (n=5) and controls (n=5). Gene expression was measured using real-time PCR, in vitro lipolysis was measured using radiolabeled oleate, and perilipin 3 (PLIN3) protein content was measured by western blotting analysis. Results: The expression of PLIN1, PLIN3, and PLIN5, along with coatomers ARF1, ARFRP1, and beta COP was similar to 80% lower in women with PCOS (all P < 0.05). Following exercise training, PLIN3 was the only perilipin to increase significantly (P! 0.05), along with coatomers ARF1, ARFRP1, bCOP, and SEC23A (all P < 0.05). Furthermore, PLIN3 protein expression was undetectable in the cell cultures from women with PCOS vs controls. Following exercise training, in vitro adipose oleate oxidation, glycerol secretion, and PLIN3 protein expression were increased, along with reductions in triglyceride content and absence of large lipid droplet morphology. Conclusions: These findings suggest that PLIN3 and coatomer GTPases are important regulators of lipolysis and triglyceride storage in the adipose tissue of women with PCOS. European Journal of Endocrinology
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