Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 3, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2015.00029
Keywords
PCR; isothermal amplification; electrical biosensor; pH monitoring; field-effect transistor
Funding
- MEXT of Japan [2306]
- ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan)
- Center of Innovation (COI) Program from Japan Science and Technology (JST) Agency
- Grants-in-Aid for Scientific Research [26107705] Funding Source: KAKEN
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Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing. Electrical and electrochemical monitoring of the amplification process draws attention over conventional optical methods because of the amenability toward point-of-care applications as there is a growing demand for nucleic acid sensing in situations outside the laboratory. A number of electrical and electrochemical techniques coupled with various amplification methods including isothermal amplification have been reported in the last 10 years. In this review, we highlight recent developments in the electrical and electrochemical monitoring of nucleic acid amplification.
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