Journal
ANALYTICAL LETTERS
Volume 48, Issue 1, Pages 89-99Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2014.930870
Keywords
Raman spectra; beta-cyclodextrin; Polyethyleneimine; Square wave voltammetry
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Funding
- Romanian National Authority for Scientific Research CNCS-UEFISCDI [PN-II-IDPCE-2011-3-0355]
- European Social Fund, Human Resources Development Operational Programme [POSDRU/159/1.5/136893]
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The simultaneous determination of ascorbic acid and uric acid has a major importance in biomedical and pharmaceutical science. A glassy carbon electrode modified with beta-cyclodextrin entrapped in polyethyleneimine was developed for the determination of ascorbic and uric acids in binary mixtures using square wave voltammetry. In order to characterize the electrode modification, Raman spectra were obtained. The modified electrode was successfully applied for the determination of ascorbic and uric acid in urine with good recoveries. The limit of detection for ascorbic acid was 0.22 mu M.
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