Journal
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
Volume 12, Issue 1, Pages 80-94Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TBCAS.2017.2759220
Keywords
Biosensor; cardiomyocytes; cell-based assay; drug screening; extracellular potential recording; impedance measurement; optical detection; stimulation
Funding
- Semiconductor Research Corporation [2484.001]
- National Science Foundation [1454555, 1610677]
- National Science Foundation Graduate Research Fellowship Program [DGE-1148903]
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [1610677] Funding Source: National Science Foundation
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [1454555] Funding Source: National Science Foundation
Ask authors/readers for more resources
This paper presents a fully integrated CMOS multimodality joint sensor/stimulator array with 1024 pixels for real-time holistic cellular characterization and drug screening. The proposed system consists of four pixel groups and four parallel signal-conditioning blocks. Every pixel group contains 16 x 16 pixels, and each pixel includes one 28 mu m x 28 mu m gold-plated electrode, four 12 mu m x 12 mu m photodiodes, and in-pixel circuits, within a 58 mu m x 58 mu m pixel footprint. Each pixel supports real-time extracellular potential recording, optical detection, charge-balanced biphasic current stimulation, and cellular impedance measurement for the same cellular sample. The proposed system is fabricated in a standard 130-nm CMOS process. Rat cardiomyocytes are successfully cultured on-chip. Measured high-resolution optical opacity images, extracellular potential recordings, biphasic current stimulations, and cellular impedance images demonstrate the unique advantages of the system for holistic cell characterization and drug screening. Furthermore, this paper demonstrates the use of optical detection on the on-chip cultured cardiomyocytes to real-time track their cyclic beating pattern and beating rate.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available