Journal
JALA
Volume 20, Issue 1, Pages 17-24Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/2211068214549625
Keywords
optofluidics; microfluidics; label-free detection; absorption spectroscopy; droplet; reaction kinetics
Funding
- National Institutes of Health [1DP2OD007209-01]
- National Science Foundation
- Penn State Materials Research Institute
- Penn State Center for Nanoscale Science (MRSEC) [DMR-0820404]
- Penn State node of the NSF
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Label-free measurements of the reaction kinetics of a small sample volume are essential for efficient drug discovery, requiring methods and systems that are rapid, accurate, and cost-effective. Herein, we present an integrated optofluidic system for label-free characterization of reactions in a nanoliter reagent volume. This system contains a droplet-based microfluidic sampling section and an optical fiber-based spectroscopy detection section. By manipulating droplets containing reagents at certain concentrations at different times, quantifiable measurements via absorption spectroscopy can be made in a simple, sensitive, and high-throughput manner. We have demonstrated our system's capability by performing potency (IC50) assays of an inhibitor in a TEM-1 -lactamase (enzyme) and nitrocefin (substrate) system. This integrated platform can potentially provide an automated, label-free, and low-cost method for many other assays of reaction kinetics.
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