Journal
HETEROCYCLES
Volume 97, Issue 1, Pages 306-313Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.3987/COM-18-S(T)20
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Funding
- JSPS KAKENHI [JP17K18295]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [17K18295] Funding Source: KAKEN
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A phosphoramidite of 3'-O,4'-C-ethyleneoxy-bridged 5-methyluridine (3',4'-EoNA-T) was successfully incorporated into oligonucleotides, and their abilities to form duplexes with RNA and DNA as well as triplexes with double-stranded DNA were evaluated. The stabilities of the duplexes formed between RNA and 3',4'-EoNA-modified oligonucleotides were only slightly lower than those of the natural DNA RNA duplex, while the duplexes formed between 3',4'-EoNA-modified oligonucleotides and DNA were drastically less stable than the natural DNA-DNA duplex. Moreover, under the same conditions as the UV-melting experiments of the duplexes, 3',4'-EoNA-modified oligonucleotides were unable to form triplexes with dsDNA. These results showed that oligonucleotides containing 3',4'-EoNA-T had excellent RNA selectivity.
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