Journal
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Volume 40, Issue 4, Pages 808-817Publisher
WILEY
DOI: 10.1002/hed.25063
Keywords
carcinoma; miR-31; miR-96; miR-182; mouth; Numb
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Funding
- Ministry of Science and Technology [MOST-102-2628-B010-011-MY3]
- Taipei Veterans General Hospital [V104C-044]
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BackgroundMicroRNAs (miRNAs) play crucial roles in head and neck squamous cell carcinoma (HNSCC). This study investigates whether miR-31, miR-96, and miR-182 are involved in targeting Numb during HNSCC. MethodsThe expression of miR-31/96/182 in tumor tissues was analyzed. Reporter assay, knockdown, expression, and oncogenic analysis were carried out in cell lines. ResultsUpregulation of miR-31/96/182 was detected in tumor tissues. In addition, advanced tumors showed higher expression levels of these miRNAs. The expression of these miRNAs was upregulated after treatment with areca ingredients (P<.01 or P<.001). These miRNAs directly targeted the 3 untranslated region (UTR) sequence of the Numb gene. An increased migration and invasion of HNSCC cells was associated with the exogenous expression of miR-31/96/182 (P<.01 or P<.001), and this was reverted by expression of Numb. ConclusionThis study provides new evidence demonstrating that there is frequent and concordant upregulation of miR-31, miR-96, and miR-182 during HNSCC and these miRNAs co-target Numb.
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