Journal
CHEMICAL PAPERS
Volume 69, Issue 1, Pages 176-182Publisher
DE GRUYTER OPEN LTD
DOI: 10.1515/chempap-2015-0012
Keywords
Gluconobacter oxydans; biosensor; ethanol; nanoparticles; carbon nanotubes
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Funding
- Slovak Research and Development Agency [APVV 0282-11]
- VEGA [1/0229/12]
- [SAV-FM-EHP-2008-04-04]
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Various types of carbon nanoparticles were directly mixed with microbial cells of Gluconobacter oxydans within a 3-D bionanocomposite in order to prepare a highly sensitive ethanol biosensor with a short response time. From all carbonaceous nanomaterials tested, single-or multi-walled carbon nanotubes provided the highest sensitivity of detection (117-121 mu A cm(-2) mM(-1)), but from a practical point of view, Ketjen black 300 and 600 provide very low detection limit (2-6 mu M) and high sensitivity for the ethanol analysis (84-88 mu A cm(-2) mM(-1)) with a short response time (14-33 s). Moreover, the price of Ketjen black is a few orders of magnitude lower compared to that of carbon nanotubes. Finally, the study showed that the morphology of nanoparticles rather than their surface modification is the key element in achieving high sensitivity of ethanol detection. (C) 2014 Institute of Chemistry, Slovak Academy of Sciences
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