Journal
GREEN CHEMISTRY
Volume 20, Issue 10, Pages 2382-2391Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8gc00091c
Keywords
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Funding
- Opening Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering [KL16-06]
- Science and Technology Planning Project of Nanjing [201716035]
- Six Talent Peaks Project in Jiangsu Province [SWYY-030]
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In this study, the bio-oxidation of galactitol to the valuable o-tagatose is presented. This proposed strategy could start with the refined by-product of waste xylose mother liquor formed by chemical hydrogenation. Through a computationally guided enzyme screening approach, a robust polyol dehydrogenase (PdPDH) was rapidly identified from a massive number of candidates. When coupled with a water-forming NADH oxidase (StNOX) for environmentally benign cofactor regeneration, subsequent reaction optimization facilitated the complete transformation of 100 g L-1 galactitol into the desired product within 15 h, with a yield of 91% in a 2 L scale preparative reaction. Compared to the current enzymatic isomerization system, our approach avoids low conversion, high operative temperatures and by-product formation, while enabling simplified product isolation.
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