Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 91, Issue 4, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiv028
Keywords
syntrophy; beta-oxidization; anaerobic digestion; quantitative PCR (qPCR); high-throughput sequencing; Syntrophomonas; Syntrophus; methanogenesis; long-chain fatty acids (LCFA)
Categories
Funding
- Biogas Research Center at Linkoping University
- US National Science Foundation Graduate Research Fellowship [DGE-1256082]
- University of Washington Valle Scandinavian Exchange Fellowship
Ask authors/readers for more resources
The ecophysiology of long-chain fatty acid-degrading syntrophic beta-oxidizing bacteria has been poorly understood due to a lack of quantitative abundance data. Here, TaqMan quantitative PCR (qPCR) assays targeting the 16S rRNA gene of the known mesophilic syntrophic beta-oxidizing bacterial genera Syntrophomonas and Syntrophus were developed and validated. Microbial community dynamics were followed using qPCR and Illumina-based high-throughput amplicon sequencing in triplicate methanogenic bioreactors subjected to five consecutive batch feedings of oleic acid. With repeated oleic acid feeding, the initial specific methane production rate significantly increased along with the relative abundances of Syntrophomonas and methanogenic archaea in the bioreactor communities. The novel qPCR assays showed that Syntrophomonas increased from 7 to 31% of the bacterial community 16S rRNA gene concentration, whereas that of Syntrophus decreased from 0.02 to less than 0.005%. High-throughput amplicon sequencing also revealed that Syntrophomonas became the dominant genus within the bioreactor microbiomes. These results suggest that increased specific mineralization rates of oleic acid were attributed to quantitative shifts within the microbial communities toward higher abundances of syntrophic beta-oxidizing bacteria and methanogenic archaea. The novel qPCR assays targeting syntrophic beta-oxidizing bacteria may thus serve as monitoring tools to indicate the fatty acid beta-oxidization potential of anaerobic digester communities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available