Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 115, Issue -, Pages 105-112Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2017.11.008
Keywords
8-iso-PGF(2 alpha)/PGF(2 alpha) ratio; F-2-isoprostanes; Oxidative stress; Inflammation; Biomarkers; Lipid peroxidation
Funding
- National Institutes of Health, National Institute of Environmental Health Sciences
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It is widely accepted that free radicals in tobacco smoke lead to oxidative stress and generate the popular lipid peroxidation biomarker 8-iso-prostaglandin F-2 alpha (8-iso-PGF(2 alpha)). However, 8-iso-PGF(2 alpha) can simultaneously be produced in vivo by the prostaglandin-endoperoxide synthases (PGHS) induced by inflammation. This inflammation-dependent mechanism has never been considered as a source of elevated 8-iso-PGF(2 alpha) in tobacco smokers. The goal of this study is to quantify the distribution of chemical-and PGHS-dependent 8-iso-PGF(2 alpha) formation in the plasma of tobacco smokers and non-smokers. The influences of gender and hormonal contraceptive use were accounted for. The distribution was determined by measuring the 8-iso-PGF(2 alpha)/prostaglandin F-2 alpha (PGF(2 alpha)) ratio. When comparing smokers (n = 28) against non-smokers (n = 30), there was a statistically significant increase in the 8-iso-PGF(2 alpha) concentration. The source of this increased 8-iso-PGF(2 alpha) was primarily from PGHS. When stratifying for gender, the increase in 8-iso-PGF(2 alpha) in male smokers (n = 9) was primarily from PGHS. Interestingly, female smokers on hormonal contraceptives had increased 8-iso-PGF(2 alpha) in both pathways, whereas those not on hormonal contraceptives did not have increased 8-iso-PGF(2 alpha). In conclusion, increased plasma 8-iso-PGF(2 alpha) in tobacco smokers has complex origins, with PGHS-dependent formation as the primary source. Accounting for both pathways provides a definitive measurement of both oxidative stress and inflammation.
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