Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 121, Issue -, Pages 26-37Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2018.04.562
Keywords
Inorganic mercury; Multicoloured fluorescent indicators; Live-cell imaging; In vivo imaging; Oxidative damage
Funding
- National Key Research and Development Program of China [2017YFC0906900, 2017YFA050400]
- NSFC [31722033, 91649123, 31671484, 31225008, 31470833]
- Shanghai Science and Technology Commission [14XD1401400, 16430723100, 15YF1402600]
- Young Elite Scientists Sponsorship Program by Cast [YESS20150027]
- Shanghai Young Top-notch Talent
- State Key Laboratory of Bioreactor Engineering
- Fundamental Research Funds for the Central Universities
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Engineered fluorescent indicators for visualizing mercury ion (Hg2+) are powerful tools to illustrate the intracellular distribution and serious toxicity of the ion. However, the sensitive and specific detection of Hg2+ in living cells and in vivo is challenging. This paper reported the development of fluorescent indicators for Hg2+ in green or red color by inserting a circularly permuted fluorescent protein into a highly mercury-specific repressor. These sensors provided a rapid, sensitive, specific, and real-time read-out of Hg2+ dynamics in solutions, bacteria, subcellular organelles of mammalian cells, and zebrafish, thereby providing a useful new method for Hg2+ detection and bioimaging. In conjunction with the hydrogen peroxide sensor HyPer, we found mercury uptake would trigger subcellular oxidative events at the single-cell level, and provided visual evidence of the causality of mercury and oxidative damage. These sensors would paint the landscape of mercury toxicity to cell functions.
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