Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 122, Issue -, Pages 202-220Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2018.04.005
Keywords
Reactive Oxygen Species (ROS); Redox signalling; Plant; in vivo detection; Fluorescent probes; Protein biosensors
Funding
- Spanish Ministry of Economy and Competitiveness [AGL2014-53771-R]
- Marie Sklodowska-Curie Action COFUND 2015 (EU project) [713366 - InterTalentum]
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Reactive oxygen species (ROS) are by-products of aerobic metabolism, and excessive production can result in oxidative stress and cell damage. In addition, ROS function as cellular messengers, working as redox regulators in a multitude of biological processes. Understanding ROS signalling and stress responses requires methods for precise imaging and quantification to monitor local, subcellular and global ROS dynamics with high selectivity, sensitivity and spatiotemporal resolution. In this review, we summarize the present knowledge for in vivo plant ROS imaging and detection, using both chemical probes and fluorescent protein-based biosensors. Certain characteristics of plant tissues, for example high background autofluorescence in photosynthetic organs and the multitude of endogenous antioxidants, can interfere with ROS and redox potential detection, making imaging extra challenging. Novel methods and techniques to measure in vivo plant ROS and redox changes with better selectivity, accuracy, and spatiotemporal resolution are therefore desirable to fully acknowledge the remarkably complex plant ROS signalling networks.
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