Journal
FORENSIC TOXICOLOGY
Volume 36, Issue 2, Pages 304-312Publisher
SPRINGER
DOI: 10.1007/s11419-018-0404-2
Keywords
EG-018; Synthetic cannabinoid; Hepatocyte metabolism; High-resolution mass spectrometry
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Funding
- Intramural Research Program of the National Institute on Drug Abuse, National Institutes of Health
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Purpose The present study aims to recommend appropriate urinary marker metabolites for documenting EG-018 consumption by investigating its metabolism in human hepatocytes. Methods For metabolite profiling, 10 mu M EG-018 was incubated in human hepatocytes for 3 h. Metabolite identification in hepatocyte samples was accomplished with high-resolution mass spectrometry via information-dependent data acquisition. Results EG-018 was highly metabolized in human hepatocytes. A total of eight metabolites were characterized, mainly generated from hydroxylation and carbonylation on the pentyl chain. Dihydrodiol formation, N-dealkylation, and glucuronidation of hydroxylated metabolites were the other major pathways. Conclusions The primary metabolites of EG-018 in human hepatocyte incubation were pentyl hydroxylated EG-018 (M6) and pentyl carbonylated EG-018 (M8). These two metabolites are proposed as the best urinary markers for confirming EG-018 intake.
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