Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 27, Issue 5, Pages 1361-1368Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-018-0389-y
Keywords
Extraction; Lipid; Microalgae; Osmotic shock; Ultrasound
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Funding
- Ministry of Research, Technology and Higher Education
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In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10-30%, solvent:biomass ratio of 5-15v/w, and extraction times of 20-50min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.
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