Journal
FOOD RESEARCH INTERNATIONAL
Volume 103, Issue -, Pages 273-279Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2017.10.054
Keywords
Soybean soluble polysaccharide; Genistein; Absorption; First-pass metabolism; Small intestine
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Funding
- National Natural Science Foundation of China [31671823]
- Development Program for Innovative Research Team of Shaanxi Normal University, China [GK201501006]
- Science and Technology Innovation as a Whole Plan Projects of Shaanxi Province, China [2015KTCQ02-01]
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This study was designed to probe the promoting effects of soybean soluble polysaccharide (SSPS) on bioavailability of genistein in mice and the underlying molecular mechanism. Male Kunming mice (n = 8) were administered intragastrically with either saline, SSPS (5 mg/kg bw), genistein (100 mg/kg bw), or SSPS (5 or 50 mg/kg bw) together with genistein (100 mg/kg bw) for consecutive 28 days. UPLC-qTOF/MS analysis showed that co-administration of SSPS and genistein in mice caused significant elevation in the urinary levels of genistein and its metabolites (p < 0.05). Furthermore, the fecal excretion of genistein was also enhanced by co-administration of SSPS. However, the feces level of dihydrogenistein, a characteristic metabolite of genistein degraded by gut microorganism, was dose-dependently decreased by the combined treatment of SSPS. Additionally, co-treatment of SSPS with genistein also decreased the small intestinal levels of uridinediphosphate-glucuronosyltransferase (UGT), sulfotransferase (SULT), P-glycoprotein (P-gp), multidrug resistance-associated protein-1 (MRP1), and multidrug resistance-associated protein-2 (MRP2) in mice. These findings suggest that the inhibition of SSPS against small intestinal first-pass metabolism of genistein is involved in the promoting effect of genistein bioavailability in mice.
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