Journal
FOOD CONTROL
Volume 92, Issue -, Pages 430-436Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2018.05.013
Keywords
Ochratoxin A; One-step immunoassay; Nanobody; Alkaline phosphatase; Polyvinylidene fluoride
Categories
Funding
- National Natural Science Foundation of China [31760493]
- Scientific Research Foundation of Hainan University [kyqd1631]
Ask authors/readers for more resources
In this work, a polyvinylidene fluoride (PVDF) membrane-based dot immunoassay was developed using a nanobody-alkaline phosphatase (Nb-AP) fusion protein for one-step, qualitative, and visual detection of ochratoxin A in cereals. Using the optimal assay conditions, the assay was completed in 6 min and the cut-off limit of this method, assessed by the naked eye, was 5 mu/kg for ochratoxin A. This method was designed to be simple, fast, and low cost without time-consuming sample preparation and cleanup procedures. Good accuracy and reproducibility were obtained in a spike-and-recovery experiment. Sample analysis results of the Nb-AP-based dot immunoassay and LC-MS/MS were in good agreement with each other. These results demonstrated that the Nb-AP-based dot immunoassay could be an acceptable method for one-step and visual detection of ochratoxin A in cereal without the need for special instruments. (C) 2018 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available