Thermal inactivation kinetics of Paenibacillus sanguinis 2301083PRC and Clostridium sporogenes JCM1416MGA in full and low fat requeijao cremoso

In this study, the thermal inactivation kinetics of Paenibacillus sanguinis 2301083PRC and Clostridium sporogenes JCM1416MGA were determined in full and low fat requeijao cremoso. Requeijdo cremoso is a type of processed cheese widely produced and consumed in Brazil. From a total of 79 strains of six different species of sporeforming bacteria isolated from requeijao cremoso, 12 strains of P. sanguinis,14 strains of B. thuringiensis, and 21 strains of C sporogenes survived to heat shock at 140 degrees C/1 min. Then, two strains (P. sanguinis 2301083PRC and C. sporogenes JCM1416MGA) were selected for the determination of inactivation kinetic parameters in full and low fat requeijao cremoso in the range of 85 degrees C-115 degrees C. The inactivation curves did not follow first order kinetic and Weibull model was used to estimate inactivation kinetic parameters. Significantly higher 8 values were found for P. sanguinis 2301083PRC in low fat requeijao cremoso compared to full fat requeijao cremoso at 85 degrees C and 105 degrees C, showing that the formulation affected heat inactivation kinetics of this microorganism (p < 0.05). C sporogenes JCM1416MGA was more heat resistant than P. sanguinis 2301083PRC, with delta (time for the first decimal reduction) values of 10, 3 and 1.3 min at 105, 110 and 115 degrees C, respectively. The 4D times (time for four decimal reductions) for this strain reached 19 min at 115 degrees C. The z values of P. sanguinis 2301083PRC and C sporogenes JCM1416MGA were 8.1 degrees C and 14.2 degrees C and 5.9 degrees C and 10.8 degrees C in low fat and full fat requeijdo cremoso, respectively. The results obtained in this study show that current thermal processes applied during requeijao cremoso production will have a minor impact on the concentration of spores of the most heat resistant strains such as C sporogenes JCM1416MGA. Despite this, C sporogenes JCM1416MGA could serve as an adequate target for the design of the thermal process to be applied in full-fat and low fat formulations of requeijao cremoso. (C) 2017 Elsevier Ltd. All rights reserved.